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Angle of deviation from the longitudinal construct axis for day-10 constructs, with perfect longitudinal alignment = 0º. <t>BMP-12</t> enhanced parallel cell alignment significantly more than control, FGF-2, TGF-β1/IGF-1, and BMP-12/FGF–2. Data points that do not share a letter are significantly different.
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Angle of deviation from the longitudinal construct axis for day-10 constructs, with perfect longitudinal alignment = 0º. BMP-12 enhanced parallel cell alignment significantly more than control, FGF-2, TGF-β1/IGF-1, and BMP-12/FGF–2. Data points that do not share a letter are significantly different.

Journal: Journal of Tissue Engineering

Article Title: Optimizing growth factor induction of tenogenesis in three-dimensional culture of mesenchymal stem cells

doi: 10.1177/2041731419848776

Figure Lengend Snippet: Angle of deviation from the longitudinal construct axis for day-10 constructs, with perfect longitudinal alignment = 0º. BMP-12 enhanced parallel cell alignment significantly more than control, FGF-2, TGF-β1/IGF-1, and BMP-12/FGF–2. Data points that do not share a letter are significantly different.

Article Snippet: FGF-2 and IGF-1 were from BioVision (San Francisco, CA), TGF-β1 was from R&D Systems (Minneapolis, MA), and BMP-12 was purchased from Sigma-Aldrich (St. Louis, MI).

Techniques: Construct

Gene expression profiles of tendon marker genes scleraxis (a), collagen types I and III (b), and decorin and biglycan (c). BMP-12 and BMP-12/IGF-1 constructs consistently increased expression of all markers on day 10. Data are reported as fold change with respect to a tendon reference control. Data points that do not share a letter are significantly different.

Journal: Journal of Tissue Engineering

Article Title: Optimizing growth factor induction of tenogenesis in three-dimensional culture of mesenchymal stem cells

doi: 10.1177/2041731419848776

Figure Lengend Snippet: Gene expression profiles of tendon marker genes scleraxis (a), collagen types I and III (b), and decorin and biglycan (c). BMP-12 and BMP-12/IGF-1 constructs consistently increased expression of all markers on day 10. Data are reported as fold change with respect to a tendon reference control. Data points that do not share a letter are significantly different.

Article Snippet: FGF-2 and IGF-1 were from BioVision (San Francisco, CA), TGF-β1 was from R&D Systems (Minneapolis, MA), and BMP-12 was purchased from Sigma-Aldrich (St. Louis, MI).

Techniques: Expressing, Marker, Construct

Glycosaminoglycan levels of day-10 constructs were assessed using the 1,9 dimethylmethylene blue dye assay. Synergistic effects of dual factor groups were detected for GAG. BMP-12 combined with IGF-1, synergistically increased GAG levels. Endpoint GAG content in the BMP-12/FGF-2 constructs was the highest at harvest, significantly greater than all single factor groups and control. Data points that do not share a letter are significantly different.

Journal: Journal of Tissue Engineering

Article Title: Optimizing growth factor induction of tenogenesis in three-dimensional culture of mesenchymal stem cells

doi: 10.1177/2041731419848776

Figure Lengend Snippet: Glycosaminoglycan levels of day-10 constructs were assessed using the 1,9 dimethylmethylene blue dye assay. Synergistic effects of dual factor groups were detected for GAG. BMP-12 combined with IGF-1, synergistically increased GAG levels. Endpoint GAG content in the BMP-12/FGF-2 constructs was the highest at harvest, significantly greater than all single factor groups and control. Data points that do not share a letter are significantly different.

Article Snippet: FGF-2 and IGF-1 were from BioVision (San Francisco, CA), TGF-β1 was from R&D Systems (Minneapolis, MA), and BMP-12 was purchased from Sigma-Aldrich (St. Louis, MI).

Techniques: Construct